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vr2332 na reference strain  (ATCC)


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    ATCC vr2332 na reference strain
    Experimental design.
    Vr2332 Na Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 169 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vr2332 na reference strain/product/ATCC
    Average 96 stars, based on 169 article reviews
    vr2332 na reference strain - by Bioz Stars, 2026-04
    96/100 stars

    Images

    1) Product Images from "Broad Protection of Pigs against Heterologous PRRSV Strains by a GP5-Mosaic DNA Vaccine Prime/GP5-Mosaic rVaccinia (VACV) Vaccine Boost"

    Article Title: Broad Protection of Pigs against Heterologous PRRSV Strains by a GP5-Mosaic DNA Vaccine Prime/GP5-Mosaic rVaccinia (VACV) Vaccine Boost

    Journal: Vaccines

    doi: 10.3390/vaccines8010106

    Experimental design.
    Figure Legend Snippet: Experimental design.

    Techniques Used:

    Phylogenetic analysis of the GP5 Amino acid sequence of the two Mosaic sequences, MN184C and VR2332. The analysis was done via the neighbor-joining method using MEGA7.0.
    Figure Legend Snippet: Phylogenetic analysis of the GP5 Amino acid sequence of the two Mosaic sequences, MN184C and VR2332. The analysis was done via the neighbor-joining method using MEGA7.0.

    Techniques Used: Sequencing

    Vaccination-induced humoral and cellular responses. ( A ) The virus neutralization was expressed as viral copy numbers (log10 scale), as measured by RT-qPCR in cell supernatants after the infection of MARC-145 cells with pre-incubated serum–virus mixtures. ( B ) The expression of IFN-γ mRNA as fold changes, by either VR2332 or MN184C-stimulated PBMCs collected on the challenge day. Each dot represents the value of one animal. The variation is expressed as standard error of the means. There were three independent replications. Significant differences were calculated by a two-way ANOVA or Student’s t test (* p < 0.05, *** p < 0.0001).
    Figure Legend Snippet: Vaccination-induced humoral and cellular responses. ( A ) The virus neutralization was expressed as viral copy numbers (log10 scale), as measured by RT-qPCR in cell supernatants after the infection of MARC-145 cells with pre-incubated serum–virus mixtures. ( B ) The expression of IFN-γ mRNA as fold changes, by either VR2332 or MN184C-stimulated PBMCs collected on the challenge day. Each dot represents the value of one animal. The variation is expressed as standard error of the means. There were three independent replications. Significant differences were calculated by a two-way ANOVA or Student’s t test (* p < 0.05, *** p < 0.0001).

    Techniques Used: Virus, Neutralization, Quantitative RT-PCR, Infection, Incubation, Expressing

    Virus clearance in sera and tissues. ( A ) Viral copy numbers in serum from 0 to 14 DPC upon VR2332 challenge. ( B ) The viral copy numbers in serum from 0 to 14 DPC upon MN184C challenge. Each dot represents the mean value of each group. The variation bars are expressed as the standard error of the mean. Three separate experiments were performed for each. A two-way ANOVA or Student’s t test (* p < 0.05, ** p < 0.01, was used to calculate significant differences; days post-challenge (DPC). ( C ) The viral copy numbers in tissues at necropsy upon VR2332 challenge. ( D ) The viral copy numbers in tissues at necropsy upon MN184C challenge. Each bar represents the mean value of each group. The bars are the standard error of the mean. Three separate experiments were performed for each. Significant differences were calculated by Student’s t test (* p < 0.05).
    Figure Legend Snippet: Virus clearance in sera and tissues. ( A ) Viral copy numbers in serum from 0 to 14 DPC upon VR2332 challenge. ( B ) The viral copy numbers in serum from 0 to 14 DPC upon MN184C challenge. Each dot represents the mean value of each group. The variation bars are expressed as the standard error of the mean. Three separate experiments were performed for each. A two-way ANOVA or Student’s t test (* p < 0.05, ** p < 0.01, was used to calculate significant differences; days post-challenge (DPC). ( C ) The viral copy numbers in tissues at necropsy upon VR2332 challenge. ( D ) The viral copy numbers in tissues at necropsy upon MN184C challenge. Each bar represents the mean value of each group. The bars are the standard error of the mean. Three separate experiments were performed for each. Significant differences were calculated by Student’s t test (* p < 0.05).

    Techniques Used: Virus

    Virus clearance in bronchioalveolar lavage fluids (BAL) and PAMs. ( A ) The viral copy numbers in BAL fluids at necropsy upon challenge with VR2332. ( B ) The viral copy numbers in BAL fluids at necropsy upon challenge with MN184C. Each bar represents the mean value of each group. Bars represent the standard error of the mean. Three independent experiments were performed for each. Significant differences were calculated by Student’s t test (* p < 0.05). ( C ) The viral copy numbers in PAMs at necropsy upon challenge with VR2332. ( D ) The viral copy numbers in PAMs at necropsy upon challenge with MN184C. Each dot represents the mean value of each animal. The bars represent the standard error of the mean. Three independent experiments were performed for each. Student’s t test was used to calculate significant differences (* p < 0.05, ** p < 0.01).
    Figure Legend Snippet: Virus clearance in bronchioalveolar lavage fluids (BAL) and PAMs. ( A ) The viral copy numbers in BAL fluids at necropsy upon challenge with VR2332. ( B ) The viral copy numbers in BAL fluids at necropsy upon challenge with MN184C. Each bar represents the mean value of each group. Bars represent the standard error of the mean. Three independent experiments were performed for each. Significant differences were calculated by Student’s t test (* p < 0.05). ( C ) The viral copy numbers in PAMs at necropsy upon challenge with VR2332. ( D ) The viral copy numbers in PAMs at necropsy upon challenge with MN184C. Each dot represents the mean value of each animal. The bars represent the standard error of the mean. Three independent experiments were performed for each. Student’s t test was used to calculate significant differences (* p < 0.05, ** p < 0.01).

    Techniques Used: Virus



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    vr2332 na reference strain  (ATCC)
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    ATCC vr2332 na reference strain
    Experimental design.
    Vr2332 Na Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Experimental design.

    Journal: Vaccines

    Article Title: Broad Protection of Pigs against Heterologous PRRSV Strains by a GP5-Mosaic DNA Vaccine Prime/GP5-Mosaic rVaccinia (VACV) Vaccine Boost

    doi: 10.3390/vaccines8010106

    Figure Lengend Snippet: Experimental design.

    Article Snippet: The viruses used in the study included the VR2332 NA reference strain (ATCC VR-2332) and the MN184C strain provided kindly by Drs. Kelly Lager and Kay Faaberg at U.S. Department of Agriculture-Agriculture Research Service (USDA-ARS).

    Techniques:

    Phylogenetic analysis of the GP5 Amino acid sequence of the two Mosaic sequences, MN184C and VR2332. The analysis was done via the neighbor-joining method using MEGA7.0.

    Journal: Vaccines

    Article Title: Broad Protection of Pigs against Heterologous PRRSV Strains by a GP5-Mosaic DNA Vaccine Prime/GP5-Mosaic rVaccinia (VACV) Vaccine Boost

    doi: 10.3390/vaccines8010106

    Figure Lengend Snippet: Phylogenetic analysis of the GP5 Amino acid sequence of the two Mosaic sequences, MN184C and VR2332. The analysis was done via the neighbor-joining method using MEGA7.0.

    Article Snippet: The viruses used in the study included the VR2332 NA reference strain (ATCC VR-2332) and the MN184C strain provided kindly by Drs. Kelly Lager and Kay Faaberg at U.S. Department of Agriculture-Agriculture Research Service (USDA-ARS).

    Techniques: Sequencing

    Vaccination-induced humoral and cellular responses. ( A ) The virus neutralization was expressed as viral copy numbers (log10 scale), as measured by RT-qPCR in cell supernatants after the infection of MARC-145 cells with pre-incubated serum–virus mixtures. ( B ) The expression of IFN-γ mRNA as fold changes, by either VR2332 or MN184C-stimulated PBMCs collected on the challenge day. Each dot represents the value of one animal. The variation is expressed as standard error of the means. There were three independent replications. Significant differences were calculated by a two-way ANOVA or Student’s t test (* p < 0.05, *** p < 0.0001).

    Journal: Vaccines

    Article Title: Broad Protection of Pigs against Heterologous PRRSV Strains by a GP5-Mosaic DNA Vaccine Prime/GP5-Mosaic rVaccinia (VACV) Vaccine Boost

    doi: 10.3390/vaccines8010106

    Figure Lengend Snippet: Vaccination-induced humoral and cellular responses. ( A ) The virus neutralization was expressed as viral copy numbers (log10 scale), as measured by RT-qPCR in cell supernatants after the infection of MARC-145 cells with pre-incubated serum–virus mixtures. ( B ) The expression of IFN-γ mRNA as fold changes, by either VR2332 or MN184C-stimulated PBMCs collected on the challenge day. Each dot represents the value of one animal. The variation is expressed as standard error of the means. There were three independent replications. Significant differences were calculated by a two-way ANOVA or Student’s t test (* p < 0.05, *** p < 0.0001).

    Article Snippet: The viruses used in the study included the VR2332 NA reference strain (ATCC VR-2332) and the MN184C strain provided kindly by Drs. Kelly Lager and Kay Faaberg at U.S. Department of Agriculture-Agriculture Research Service (USDA-ARS).

    Techniques: Virus, Neutralization, Quantitative RT-PCR, Infection, Incubation, Expressing

    Virus clearance in sera and tissues. ( A ) Viral copy numbers in serum from 0 to 14 DPC upon VR2332 challenge. ( B ) The viral copy numbers in serum from 0 to 14 DPC upon MN184C challenge. Each dot represents the mean value of each group. The variation bars are expressed as the standard error of the mean. Three separate experiments were performed for each. A two-way ANOVA or Student’s t test (* p < 0.05, ** p < 0.01, was used to calculate significant differences; days post-challenge (DPC). ( C ) The viral copy numbers in tissues at necropsy upon VR2332 challenge. ( D ) The viral copy numbers in tissues at necropsy upon MN184C challenge. Each bar represents the mean value of each group. The bars are the standard error of the mean. Three separate experiments were performed for each. Significant differences were calculated by Student’s t test (* p < 0.05).

    Journal: Vaccines

    Article Title: Broad Protection of Pigs against Heterologous PRRSV Strains by a GP5-Mosaic DNA Vaccine Prime/GP5-Mosaic rVaccinia (VACV) Vaccine Boost

    doi: 10.3390/vaccines8010106

    Figure Lengend Snippet: Virus clearance in sera and tissues. ( A ) Viral copy numbers in serum from 0 to 14 DPC upon VR2332 challenge. ( B ) The viral copy numbers in serum from 0 to 14 DPC upon MN184C challenge. Each dot represents the mean value of each group. The variation bars are expressed as the standard error of the mean. Three separate experiments were performed for each. A two-way ANOVA or Student’s t test (* p < 0.05, ** p < 0.01, was used to calculate significant differences; days post-challenge (DPC). ( C ) The viral copy numbers in tissues at necropsy upon VR2332 challenge. ( D ) The viral copy numbers in tissues at necropsy upon MN184C challenge. Each bar represents the mean value of each group. The bars are the standard error of the mean. Three separate experiments were performed for each. Significant differences were calculated by Student’s t test (* p < 0.05).

    Article Snippet: The viruses used in the study included the VR2332 NA reference strain (ATCC VR-2332) and the MN184C strain provided kindly by Drs. Kelly Lager and Kay Faaberg at U.S. Department of Agriculture-Agriculture Research Service (USDA-ARS).

    Techniques: Virus

    Virus clearance in bronchioalveolar lavage fluids (BAL) and PAMs. ( A ) The viral copy numbers in BAL fluids at necropsy upon challenge with VR2332. ( B ) The viral copy numbers in BAL fluids at necropsy upon challenge with MN184C. Each bar represents the mean value of each group. Bars represent the standard error of the mean. Three independent experiments were performed for each. Significant differences were calculated by Student’s t test (* p < 0.05). ( C ) The viral copy numbers in PAMs at necropsy upon challenge with VR2332. ( D ) The viral copy numbers in PAMs at necropsy upon challenge with MN184C. Each dot represents the mean value of each animal. The bars represent the standard error of the mean. Three independent experiments were performed for each. Student’s t test was used to calculate significant differences (* p < 0.05, ** p < 0.01).

    Journal: Vaccines

    Article Title: Broad Protection of Pigs against Heterologous PRRSV Strains by a GP5-Mosaic DNA Vaccine Prime/GP5-Mosaic rVaccinia (VACV) Vaccine Boost

    doi: 10.3390/vaccines8010106

    Figure Lengend Snippet: Virus clearance in bronchioalveolar lavage fluids (BAL) and PAMs. ( A ) The viral copy numbers in BAL fluids at necropsy upon challenge with VR2332. ( B ) The viral copy numbers in BAL fluids at necropsy upon challenge with MN184C. Each bar represents the mean value of each group. Bars represent the standard error of the mean. Three independent experiments were performed for each. Significant differences were calculated by Student’s t test (* p < 0.05). ( C ) The viral copy numbers in PAMs at necropsy upon challenge with VR2332. ( D ) The viral copy numbers in PAMs at necropsy upon challenge with MN184C. Each dot represents the mean value of each animal. The bars represent the standard error of the mean. Three independent experiments were performed for each. Student’s t test was used to calculate significant differences (* p < 0.05, ** p < 0.01).

    Article Snippet: The viruses used in the study included the VR2332 NA reference strain (ATCC VR-2332) and the MN184C strain provided kindly by Drs. Kelly Lager and Kay Faaberg at U.S. Department of Agriculture-Agriculture Research Service (USDA-ARS).

    Techniques: Virus